Journal:

Article Title: The murine buccal mucosa is an inductive site for priming class I-restricted CD8 + effector T cells in vivo

doi: 10.1046/j.1365-2249.1998.00671.x

Figure Lengend Snippet: Immunohistochemical analysis of dendritic cells (DC) of the buccal and skin epithelium. Epithelial sheets from the buccal mucosa (a,b,c) and the skin (d,e,f) from BALB/c mice were stained with MoAbs specific for MHC class II (CD311) (a and d), CD11c (N418) (b and e) and the mannose receptor DEC-205 (NLDC-145) (c and f). Staining is exclusively observed on cells with dendritic morphology. (Final mag. × 400.)

Article Snippet: The panel of mouse-specific MoAbs used included: a rat anti-MHC class II (CD311) [ 10 ], a rat anti-B7.1 (CD80) MoAb (clone 1G10) (ATCC, Rockville, MD), a rat anti-B7.2 (CD86) MoAb (clone GL1), a hamster anti-CD40 MoAb and FITC-conjugated anti-Fas MoAb (all from Pharmingen, Clinisciences, France), the rat anti-DEC-205 MoAb [ 11 ] (NLDC-145, a gift from G. Kraal, University, Amsterdam, The Netherlands), an anti-CD11c MoAb (N418) [ 12 ] (ATCC), a PE-conjugated anti-CD4 (GK1.5) and FITC-conjugated anti-CD8a MoAbs (YTS 169.4) (both from Caltag, Tebu, France).

Techniques: Immunohistochemical staining, Staining

Journal: Cell Reports Medicine

Article Title: Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants

doi: 10.1016/j.xcrm.2020.100095

Figure Lengend Snippet:

Article Snippet: Hamster anti-CD11c-BV786-conjugated (N418) , BD PharMingen , Cat# 563735.

Techniques: Blocking Assay, Derivative Assay, Recombinant, Protein Extraction, Staining, Electron Microscopy, Cell Culture, Enzyme-linked Immunosorbent Assay, Software

Journal: Cell reports

Article Title: PAD4 controls tumor immunity via restraining the MHC class II machinery in macrophages

doi: 10.1016/j.celrep.2024.113942

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Hamster monocloncal antibody anti-mouse CD11c (clone N418) , Invitrogen , Cat# 25-0114-82; RRID: AB_469590.

Techniques: Recombinant, Plasmid Preparation, Lysis, Protease Inhibitor, SYBR Green Assay, Western Blot, Chromatin Immunoprecipitation, Magnetic Beads, Mutagenesis, Cell Isolation, Isolation, Transfection, Enzyme-linked Immunospot, Mass Spectrometry, Expressing, Transgenic Assay, CRISPR, Software

Journal: Molecular Cell

Article Title: The ribotoxic stress response drives acute inflammation, cell death, and epidermal thickening in UV-irradiated skin in vivo

doi: 10.1016/j.molcel.2024.10.044

Figure Lengend Snippet:

Article Snippet: PE-Cyanine7 Hamster anti-CD11c clone N418 , Invitrogen , #25-0114-81; RRID: AB_469589.

Techniques: Recombinant, Reverse Transcription, In Situ, Knock-Out, Variant Assay, Software, Purification, Blocking Assay

Journal: Molecular Cell

Article Title: The ribotoxic stress response drives acute inflammation, cell death, and epidermal thickening in UV-irradiated skin in vivo

doi: 10.1016/j.molcel.2024.10.044

Figure Lengend Snippet:

Article Snippet: PE-Cyanine7 Hamster anti-CD11c clone N418 , Invitrogen , #25-0114-81; RRID: AB_469589.

Techniques:

Journal: Scientific Reports

Article Title: Ablation of CD8α + dendritic cell mediated cross-presentation does not impact atherosclerosis in hyperlipidemic mice

doi: 10.1038/srep15414

Figure Lengend Snippet: ( a ) Total RNA was isolated from fresh-frozen human atherosclerotic plaques. Real-time PCR results of expression levels of BDCA3, IRF8, ADFP, Batf3, TAP1 and Necl2 are shown as mean ± SEM. All expression levels were first normalized for levels of β-actin expression, and are depicted as fold induction when compared to expression levels in early plaques. Samples were grouped based on histological qualification of plaque stage according to Virmani et al . . Early: Intimal Thickening/ Pathological Intimal Thickening (n = 5), Advanced: Thick/Thin Fibrous Cap Atheroma (n = 6), Unstable: Intra Plaque Hemorrhage (n = 5). *p < 0.05, ***p < 0.001. ( b ) Representative images of frozen human carotid plaque sections (n = 8–10) doublestained with antibodies against XCR1 (green) and CD11c (red) to identify cross-presenting DCs. Colocalization was determined using a Nuance Spectral Imaging System and is indicated in yellow. ( c ) Total RNA was isolated from fresh-frozen mouse aorta’s. Real-time PCR results of expression levels of Rab11b, TAP1 and XCR1 are shown as mean ± SEM. All expression levels were first normalized for levels of GAPDH expression, and are depicted as fold induction when compared to expression levels in early plaques. Early: 8 wk old C57Bl6 mice (n = 6), Advanced: >35 wk old C57Bl6 ApoE −/− mice (n = 5) ( d ) Representative images of frozen mouse aortic root sections doublestained with antibodies against CD8α (red) and CD11c (blue) to identify cross-presenting DCs. Nuclei were lightly counterstained with MethylGreen. Arrow: doublestained cell.

Article Snippet: Immunohistochemical stainings were performed on paraffin or frozen aortic root sections for CD3 (DAKO, Glostrup, Denmark), α‐smooth muscle actin (ASMA) (DAKO), Mac3 (BD), cleaved caspase 3 (Cell Signaling), CD11c (supernatant of N418 Hybridoma Cells), CD8α (Thermo Scientific), biotinylated CD45.1 (BD Biosciences) or biotinylated CD45.2 (BD Biosciences).

Techniques: Isolation, Real-time Polymerase Chain Reaction, Expressing, Imaging

Journal: Scientific Reports

Article Title: Ablation of CD8α + dendritic cell mediated cross-presentation does not impact atherosclerosis in hyperlipidemic mice

doi: 10.1038/srep15414

Figure Lengend Snippet: ( a ) Lethally irradiated ldlr −/− mice were reconstituted with wt (n = 15) or batf3 −/− (n = 12) bone marrow, and after 6 weeks recovery, put on a WTD containing 0,25% cholesterol for 10 weeks. ( b ) Representative flow cytometry gating of CD8α + DC population (Lin − , CD11c high , MHCII high , CD8α + ). ( c ) Bar graph of CD8α + DCs as percentage of cDCs. ( d ) Bar graph of CD103 + DCs as percentage of cDCs. ( e ) Body weight at sacrifice. ( f ) Total cholesterol content in serum at sacrifice. Data are presented as mean ± SEM, **p < 0,01, ***p < 0,001.

Article Snippet: Immunohistochemical stainings were performed on paraffin or frozen aortic root sections for CD3 (DAKO, Glostrup, Denmark), α‐smooth muscle actin (ASMA) (DAKO), Mac3 (BD), cleaved caspase 3 (Cell Signaling), CD11c (supernatant of N418 Hybridoma Cells), CD8α (Thermo Scientific), biotinylated CD45.1 (BD Biosciences) or biotinylated CD45.2 (BD Biosciences).

Techniques: Irradiation, Flow Cytometry